Drug-specific in vitro release of IFN-gamma in patients with delayed cutaneuos drug hypersensitivity reactions
نویسندگان
چکیده
Method A total of 16 patients with clinically well-established maculopapular exanthema due to antiepileptic drugs hypersensitivity in remission state and 15 drug-exposed control donors without DHR were included to the study. Peripheral blood mononuclear cells of investigated individuals were isolated and cultured under defined conditions with drugs. IFN-gamma production was measured with electrochemiluminescence array assay and ELISA (cytokine level in cell culture supernatant), ELISpot (cytokine secreting cells), flow cytometry (intracellular staining in CD3+ CD4+ cells).
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